By Lester Packer (Eds.)

ISBN-10: 0121821153

ISBN-13: 9780121821159

The severely acclaimed laboratory regular, Methods in Enzymology, is without doubt one of the so much hugely revered guides within the box of biochemistry. in view that 1955, each one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate today--truly a vital booklet for researchers in all fields of existence sciences

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Additional info for Carotenoids Part B: Metabolism, Genetics, and Biosynthesis

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Kuller, Cancer 56, 2593 (1985). 4A. Vahlquist, K. Carlson, D. Hallberg,and S. Rossner,Int. J. Obes. 6, 491 (1982). s M. M. Mathews-Rothand C. L. Gulbrandsen, Clin. Chem. 20, 1578(1974). 6L. K. Bjornson, H. J. Kayden, E. Miller, and A. N. Moshell,J. LipidRes. 17, 343 (1976). 7p. p. Reddy,B. A. Clevidence,E. Berlin, P. R. Taylor, J. G. Bieri, and J. C. Smith, FASEB J. )(1989). METHODS IN ENZYMOLDGY, VOL. 214 Copyright© 1993 by Academic Pre~ Inc. All rights of reproduction in any form reserved. 34 METABOLISM [4] lated, human LDL, suggesting a strong affinity of carotenoids for this lipoprotein class.

Aponte, and G. Wolf, J. Nutrit. Biochem. 3, 118 (1992). 26 METABOLISM [3] 38O • ' ' retinol 280 "0 180 X-. 5 ml) FIo. 2. Chromatographic analysis of [14C]retinol and p%]retinoic acid produced from p4C]BC by cells. ['4C]BC (1 nmol) was mixed with 1 ml of culture medium and incubated in pl~sence (O) or in absence (@) of confluent hBRIE 380 cells at 37 °. After 24 hr the cells or an aliquot of the medium were analyzed for BC cleavage products as described in the text. (Reproduced with permission from Ref.

Various apolipoproteins serve as recognition ligands for lipoprotein receptors on tissues, enabling lipoprotein uptake and catabolism at specific sites. The apolipoprotein components also serve as cofactors for certain enzymes, such as hepatic and extrahepatic lipases, which are inti= mately involved in lipoprotein metabolism and hence in regulating transfer of lipoprotein components to tissues. Thus, it is reasonable to infer that the final destination and ultimate metabolic fate of a carotenoid depends largely on the relative al~nity of that carotenoid for specific lipoprotein classes.

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Carotenoids Part B: Metabolism, Genetics, and Biosynthesis by Lester Packer (Eds.)


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