By Teresa V. Bowman, Akil A. Merchant (auth.), Meral Beksac (eds.)
Molecular equipment: Stem cellphone Transplantation offers a compendium of state-of-the-art study at the molecular steps fascinated with hematopoietic stem mobile (HSC) activation and self-renewal. The emergence of HLA typing, and the optimistic effect it has had at the good fortune of scientific transplantation is emphasised and mentioned through awesome stem phone researchers.
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Extra resources for Bone Marrow and Stem Cell Transplantation
Kim, Y. , Gambhir, S. , and Witte, O. N. (2004) Multimodality imaging of lymphocytic migration using lentiviral-based transduction of a tri-fusion reporter gene. Mol. Imaging Biol. 6, 331–340. , and Germain, R. N. (2002) Imaging of T-cell interactions with antigen presenting cells in culture and in intact lymphoid tissue. Immunol. Rev. 189, 51–363. Sipkins, D. , Wu, J. , et al. (2005) In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment. Nature 435, 969– 973.
Nucl. Med. Biol. 31, 93–101. Hildebrandt, I. J. and Gambhir, S. S. (2004) Molecular imaging applications for immunology. Clin. Immunol. 111, 210–224. , et al. (2004) Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord. J. Neurosci. Res. 76, 232–243. , et al. (2002) Tracking transplanted stem cell migration using bifunctional, contrast agent-enhanced, magnetic resonance imaging. Neuroimage 17, 803–811. , et al.
5 mL of PBS. Do not store. 3. 1. Retroviral Transduction of Murine Bone Marrow and Stem Cells DAY 1: 1. Inject 250 mg/kg 5FU intraperitoneally or intravenously into each donor mouse (see Note 4). DAY 3: 2. Split packaging cell line for transfection per protocol. 5 ϫ 106 cells per 6-cm dish. Incubate at 37°C/5% CO2 overnight. 28 Duda et al. DAY 4: 3. Transfect packaging cell line per protocol. For Lipofectamine 2000, prepare DNA-Lipofectamine 2000 complexes, one tube for each sample: a. 5 mL of Opti-MEM I reduced serum medium.
Bone Marrow and Stem Cell Transplantation by Teresa V. Bowman, Akil A. Merchant (auth.), Meral Beksac (eds.)