By Clarence H. and J. Throck Watson (editors) Suelter

ISBN-10: 0471613037

ISBN-13: 9780471613039

Discusses how mass spectrometry will be utilized to numerous organic difficulties in a fashion that's significant to readers with various levels of data concerning the topic. instead of purely describing tools, it presents insights into ways that mass spectroscopists have used to unravel those difficulties. each one bankruptcy bargains suggestions in applicable how one can isolate and purify the compound classification ahead of research or characterization by way of mass spectrometry. Chapters additionally characteristic tutorials on mass spectral interpretations in accordance with the fragmentation pathways mostly encountered for the actual compound variety. debts are given of recent methods to research in keeping with fast-atom bombardment in addition to extra traditional research by means of electron effect and chemical-ionization mass spectrometry.

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Sample text

PRESSURE MEASUREMENT Although pressure measurement seems like a simple task, 99% of mass spectrometrists have no idea of the exact pressure inside the ion source. In fairness, they have no need to know, as long as the instrument is performing properly, but the fact points out the difficulty of mass-spectrometric pressure measurement. At pressures down to about 500 Pa (4 Torr), a simple mercury manometer is a suitable pressure-measuring device. The height of a column of mercury can be measured and will be proportional to the pressure of the system, regardless of what gas is present.

Typical commercial ion traps achieve unit mass resolution up to about 650 daltons. The ion traps are simple and inexpensive, which should make them a popular choice for laboratories with a high volume of samples, such as contract environmental or clinical laboratories. At the present time, however, quadrupoles still outnumber ion traps in these settings. Ion traps are closely akin to another class of mass analyzers, the ion cyclotrons and Fourier-transform mass spectrometers. Space does not permit their discussion here, but detailed information is available in recent reviews (15,16).

The Pirani gauge is a similar device, except that the heated wire makes up one arm of a Wheatstone bridge circuit. As the temperature of the wire increases, the resistance increases. This change in resistance is detected by the Wheatstone bridge and displayed on the meter in units of pressure. The Pirani gauge is also a relative pressure gauge and covers the same range of pressures as the thermocouple gauge. For the low pressures inside an operating mass spectrometer, still another type of gauge is required.

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Biomedical applications of mass spectrometry by Clarence H. and J. Throck Watson (editors) Suelter


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