By Christophe Clement, Ettore Pacini, Jean-Claude Audran
Pollen, the plant constitution most generally utilized by people, is a key constitution in plant copy giving upward thrust to culmination and seeds. furthermore, the biotechnological use of pollen is of serious significance for plant breeders because it permits to acquire kinds with larger usage and yield. within the first half, the successive steps of pollen improvement within the anther from floral induction to pollen germination and fertilization are completely tested; the second one half is dedicated to pollen behaviour in vitro.
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Extra resources for Anther and Pollen: From Biology to Biotechnology
1997). This resulted in the cloning of 22 cDNA clones expressed in stamen at distinct stages of development. Fourteen of these clones corresponded to genes whose expression was detected in pre-meiotic stamens, a stage of development for which very little information is presently available. Furthermore, the absence of similarities with sequences from the data base for 10 clones suggests that they represent novel genes (Table 1). In parallel, these clones are being exploited as developmental markers of early differentiation within the flower and their expression patterns are being analysed by in situ hybridisation.
1 Introduction Aliphatic polyamines are plant growth regulators that affect mainly the induction and sustenance of cell division (Bagni 1989). They are involved in all the steps of protein synthesis (replication, transcription and translation) through specific and aspecific interactions with DNA and different RNAs (Feuerstein and Marton 1989). In particular, the specific interactions of two molecules of spermine or spermidine, one located in the major groove at one end of the anticodon stem, the other near the variable loop of tRNA, are able to switch tRNA conformation from the inactive to the active form (which is then able to accept the specific amino acid) (Quigley et al.
In our collection of mutant, the largest deletion found represents more than 50% of the (p)-arm of the Y chromosome in a bisexua mutant, and 40% of the same arm in an asexua phenotype. No other abnormality than flower phenotypes was observed in these mutants. This suggests the presence of only a few genes (corresponding to probably only SPF and GSF) on the short arm of this chromosome. , pseudo-autosomal region 9'F GSF ~ ______ ______ ~ short arm ~o~ ________ __ ~ ~ long arm Fig. 3. 43 in a bisexua mutant and an asexua mutant.
Anther and Pollen: From Biology to Biotechnology by Christophe Clement, Ettore Pacini, Jean-Claude Audran